BioMed Research International

Research Article

Effects of Reactive Nitrogen Scavengers on NK-Cell-Mediated Killing of K562 Cells

Table 3

The effect of endogenous RNM on the activity of NK cells.
GroupsNO (6 h) (μmol/mL)TNF-β (24 h) pg/mLIFN-γ (24 h) pg/mLNCC (24 h) %
Control 1 1 . 6 8 ± 6 . 6 2 1 1 . 0 8 ± 5 . 4 6 1 1 . 6 5 ± 5 . 0 5
T + IL-2/PHA 2 2 . 2 9 ± 5 . 6 6 2 0 . 6 4 ± 5 . 5 7 1 3 . 1 8 ± 5 . 8 6
MO  + IL-2/PHA 1 1 4 . 3 7 ± 7 . 4 0 4 0 . 6 4 + 7 . 5 9 4 7 . 7 6 ± 6 . 5 7
E + IL-2/PHA 6 2 . 6 4 ± 7 . 0 0 3 6 1 . 6 2 ± 1 2 . 2 7 2 8 4 . 7 4 ± 7 . 4 9
E  +  MO  + IL-2/PHA 1 1 9 . 6 2 ± 1 1 . 1 8 1 1 4 . 0 9 ± 7 . 4 6 7 6 . 7 7 ± 4 . 9 9
MO  +  T  +  IL-2/PHA 1 1 5 . 2 6 ± 6 . 4 7 3 3 . 3 1 ± 6 . 3 4 4 6 . 4 6 ± 6 . 9 7
E  +  T  +  IL-2/PHA 7 9 . 6 3 ± 7 . 0 4 3 7 1 . 9 9 ± 1 2 . 7 9 2 7 5 . 0 8 ± 9 . 6 1 9 1 . 7 7 ± 3 . 6 2
E  +  T  +  MO + IL-2/PHA 1 8 9 . 3 5 ± 6 . 5 1 * 1 1 0 . 9 1 ± 1 0 . 0 1 * 7 4 . 7 4 ± 1 0 . 1 5 * 6 0 . 3 9 ± 5 . 3 9 *
𝑛 = 3 ; ONOO− 200 umol/L; DHT 20 umol/L, TIP 50 umol/L, GSH 50 umol/L; T is K562 cells, E is mononuclear enriching NK cells E/T = 10/1; * 𝑃 < 0 . 0 5 , comparison between group NK  +  MO  +  K562  +  IL-2 and group NK  +  K562  +  IL-2.