Research Article
Protein Expression of Mesenchymal Stem Cells after Transfection of pcDNA3.1−-hVEGF165 by Ultrasound-Targeted Microbubble Destruction
Table 1
Protein expression of VEGF165 in mesenchymal stem cells supernatant after transfection (, ng/mL).
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, versus each other non-UTMD groups. (1) The blank control group: MSCs with culture fluid. (2) The control group A: 4 μg pcDNA3.1−-hVEGF165 recombinant were transfected into MSCs. (3) The control group B: 4 μg pcDNA3.1−-hVEGF165 recombinant mixed with 300 μl SonoVue microbubble were transfected into MSCs. (4) The control group C: 4 μg pcDNA3.1−-hVEGF165 recombinant were transfected into MSCs by ultrasonic exposure (illustrated by the example of Mechanic index (MI) 1.0 and exposure time (ET) 60 s). (5) The UTMD group: 4μg pcDNA3.1−-hVEGF165 recombinant were transfected into MSCs by UTMD (MI 1.0, ET 60 s). |