Mechanical and Electrophysiological Properties of the Sarcolemma of Muscle Fibers in Two Murine Models of Muscle Dystrophy: Col6a1 and Mdx
Figure 4
Passive and active membrane electrical properties of single muscle fibers kept in culture. Resting membrane potential (a), maximum rate of depolarization (b), action potential amplitude (c), and resting conductance determined with a hyperpolarizing step and normalized to sarcolemma surface of the wild type (black column), Col6a1 (grey column), mdx (white column). Mean and standard errors of measurements obtained in 66 wild type, 52 Col6a1 and 32 mdx fibers. P < .05 and P < .001 compared to wild type. (e) shows the determination of membrane conductance in a separate set of wild type (black column) and Col6a1 fibers (grey column) in the absence and in the presence of 9-anthracene carboxylic acid (9- AC). denotes P < .05 both in comparison between the two fiber types and in comparison between absence and presence of 9-AC.
