Metabolic Engineering for Production of Biorenewable Fuels and Chemicals: Contributions of Synthetic Biology

Table 1

Summary of engineered E. coli biocatalysts for production of renewable fuels and chemicals in our laboratory.

Product	Fermentation condition^(1)	Titer (g/L)	Yield(g/g)	Productivity (g/L/h)	Reference
Redesign through modification of existing pathways
D-lactate	Anaerobic, batch	118	0.98	2.88	[22]
Acetate	Aerobic, fed-batch	53	0.50	1.38	[23]
Succinate	Anaerobic, batch	83	0.98	0.90	[24]
Redesign through introduction of foreign pathways
Ethanol	Anaerobic, batch	43	0.48	2.00	[25]
L-lactate	Anaerobic, batch	116	0.98	2.29	[22]
Xylitol	Aerobic, fed-batch	38	1.40	0.81	[26]
L-alanine	Anaerobic, batch	114	0.95	2.38	[27]

⁽¹⁾All fermentations were done in mineral salts medium with glucose, except for the ethanol fermentations which used xylose.