Two-Dimensional Liquid Chromatography Technique Coupled with Mass Spectrometry Analysis to Compare the Proteomic Response to Cadmium Stress in Plants
Table 1
Proteins regulated by Cd treatment in leaves of Populus nigra clone βPoliβ, separated by 2D-LC.
Homologous protei
Accession numbe
Measured pI interva
Predicted pI/Mr
Score / coverage
Rati
ESTs popla
LC1
Aquaporin
NIP12_ARATH
4.00
8.63/31249
56/40
P047H06
LC2
Dehydration-responsive protein
gi18405331
4.03β4.33
8.60/67472
60/35
P082D06
LC3
Predicted protein
gi162695261
5.11β5.41
6.20/45886
69/32
T020D11
LC4
Acetyl coenzime A carboxylase
gi74272305
5.41β5.71
5.60/120177
69/35
Q001C04
LC5
NADPH isocitrate dehydrogenase
gi169989
5.71β5.99
6.13/49508
51/28
N006H08
LC6
Thylakoidal processing peptidase 2
TPP2_ARATH
6.29β6.59
6.54/40581
56/21
T040C06
LC7
Hypothetical protein
gi125603717
6.29β6.59
6.67/47148
71/26
Y004E07
LC8
Predicted protein
gi168009327
7.49β7.79
7.22/42542
69/40
V017A06
LC9
Chaperone ClpB
CLPB_RHOBA
8.00
5.06/97498
110/56
P058E06
LC10
Rubisco large subunit
gi33636375
8.00
5.13/6207
69/80
No match
LC11
Aldo/keto reductase protein
gi15232354
8.00
6.46/35185
64/35
V056F06
LC12
Cytochrome P450
C71BT_ARATH
8.00
8.06/56318
105/64
M122H12
LC13
ATP-dependent Clp protein
CLPR1_ARATH
8.00
8.81/42942
51/35
UM61TE04
LC14
Cysteine proteinase inhibitor 2
CYT2_ORYSJ
8.00
8.82/16550
56/25
FL_GENBANK_63
LC15
Hydroxyacylglutathione hydrolase 1
GLO2M_ARATH
8.00
8.83/36933
51/40
S065B02
LC16
Hypothetical protein
gi38175624
8.00
9.10/27188
69/36
N054D02
LC17
GTP binding protein
gi145359760
8.00
9.46/20079
60/40
K027P68
LC18
Protein kinase APK1B
APK1B_ARATH
8.00
9.37/45946
56/37
N041C12
LC19
CRS2A chloroplastic intron splicing facilitator
CRS2A_ARATH
8.00
9.40/27356
69/60
K028P24
LC20
Nucleotide translocator
gi444790
8.00
9.78/43995
92/43
T027D03
aThe nos. refer to the spot numbers as given in Figure 3. bPutative protein identification and accession number for the closest match in the SWISS PROT and NCBI databases. cpI values as estimated from the 2D-LC together with the predicted pI and Mr of the closest match in the databases. dScore and percentage of coverage of the matching peptide sequence tags. eRatio between values of the peak areas of the proteins from treated leaves (50βM CdSO4) and untreated leaves (0βM CdSO4) obtained from the Deltavue software. fMatches with the poplar EST database.