Research Article
Genotoxic and Cytotoxic Safety Evaluation of Papain (Carica papaya L.) Using In Vitro Assays
Table 3
Mutoxitest—Number of Trp+ revertants/plate (mean ± SD). Aliquots (100 L) of exponentially growing cultures were mixed with 100 L of different concentrations of the papain or 0.9% NaCl, as negative control, and mixed with 2.5 mL molten top agar at 45C and plated on minimal glucose agar plates supplemented with 0.5 mg tryptophan/litre. The mutagenic responses were expressed as the absolute number of Trp+ revertants/plate, after incubation at 37C, for 48 hours. Values are the mean of 3 independent experiments with standard deviations not exceeding 15%.
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Number of H2O2-induced revertants equivalent to that found with other genotoxic agents used by Blanco et al. in 1998. when compared to negative control (0.9% NaCl). |