The Masticatory Contractile Load Induced Expression and Activation of Akt1/PKB𝛼 in Muscle Fibers at the Myotendinous Junction within Muscle-Tendon-Bone Unit

Figure 3

Immunoblot analysis of Akt/PKB in peripheral muscle fibers and in muscle cells near to the myotendinous areas. In peripheral masticatory muscle fibers and masticatory muscle fibers at MTJ, protein extracts were analyzed by immunoblot analysis using an antibody against Akt1/PKB𝛼. The specific immunoblot bands of approximately 60 kDa identify Akt1/PKB𝛼 in peripheral muscle fibers and in muscle fibers near to the MTJ. The level of Akt1/PKB𝛼 in muscle fibers near to the MTJ was higher than that in peripheral muscle fibers.