A New Caspase-8 Isoform Caspase-8s Increased Sensitivity to Apoptosis in Jurkat Cells

Figure 9

Mitochondrial membrane depolarization was assessed by the loss of DiOC6 staining of the mitochondria: flow cytometric analysis of the effect of anti-Fas mAb (CH11) treatment on mitochondrial transmembrane potential of empty vector (JP4) or caspase-8s–transfected Jurkat cells (JS2) and wild-type Jurkat cells. Cells untreated and treated with 50 ng/mL CH11 for 24 h were double stained with DiOC6 and PI. Data shown are representative of three independent experiments.