Research Article
The Cyclodextrin Glycosyltransferase of Paenibacillus pabuli US132 Strain: Molecular Characterization and Overproduction of the Recombinant Enzyme
Table 3
Purification steps of the recombinant US132
CGTase.
| | Purification step | Total activity (U) | Total protein (mg) | Specific activity (U/mg) | Yield (%) | Purification (fold) |
| | Crude extract
| 7000
| 35
| 200
| 100
| — | | Heat treatment | 6650 | 14.8 | 450 | 95 | 2.2 | | HIC* | 4340
| 1.55
| 2800
| 62
| 14
| | Starch adsorption | 1400 | 0.28 | 5000 | 20 | 25 |
|
|
*HIC: Hydrophobic Interaction Chromatography.
|
