Metabolic Control Analysis: A Tool for Designing Strategies to Manipulate Metabolic Pathways
Figure 12
Determination of flux control
coefficients in an in vitro
reconstitution of the final section of Entamoeba histolytica glycolysis.
Enzymatic assay with the three recombinant enzymes from
the ameba: EhPGAM, EhENO, and EhPPDK. LDH, commercial lactate dehydrogenase. The flux control coefficient was determined at
the *marked position. 2PG,
2-phosphoglycerate; 3PG, 3-phosphoglycerate. Modified from [143].