Research Article

Preparation and Characterization of a Novel Chimeric Protein VEGI-CTT in Escherichia coli

Figure 4

Western blotting analysis of the renatured, purified recombinant protein VEGI-CTT. Ten micrograms of VEGI-CTT were applied into 12% SDS-PAGE, and then electrotransferred to a PVDF membrane. Western blotting analysis was performed using mouse anti-His-Tag monoclonal antibody (Lane 2). The membrane was incubated with the second antibody: HRP-labeled goat antimouse IgG. Lane 1: prestained molecular weight protein markers. Lane 2: fusion protein VEGI-CTT of 22 kDa.
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