Preparation and Characterization of a Novel Chimeric Protein VEGI-CTT
in Escherichia coli
Figure 4
Western blotting analysis of the renatured, purified recombinant
protein VEGI-CTT. Ten micrograms of VEGI-CTT were
applied into 12% SDS-PAGE, and then electrotransferred to a PVDF membrane.
Western blotting analysis was performed using mouse anti-His-Tag
monoclonal antibody (Lane 2). The membrane was incubated with the
second antibody: HRP-labeled goat antimouse IgG. Lane 1: prestained
molecular weight protein markers. Lane 2: fusion protein VEGI-CTT of
22 kDa.