Direct Organogenesis from Rhizome Explants in Marsilea quadrifolia L.: A Threatened Fern Species
Table 1
Effect of different concentrations of cytokinins (BAP and Kin) on induction of shoots from rhizome explants of M. quadrifolia.
Conc. of cytokinins (mg/L)
Response (%)
Number of shoots (Mean ± SD)
Shoot length (cm) (Mean ± SD)
Control (0.0)
0.00
0.0 ± 0.00
0.00 ± 0.00
BAP
0.5
96
6.2 ± 1.39
2.72 ± 0.40
1.0
69
3.7 ± 0.51
1.60 ± 1.25
1.5
73
4.8 ± 1.04
1.33 ± 0.18
2.0
61
3.0 ± 0.73
1.10 ± 0.12
2.5
48
4.8 ± 1.04
1.33 ± 0.18
3.0
22
2.5 ± 0.30
0.44 ± 0.31
Kin
0.5
57
3.3 ± 0.26
1.31 ± 0.22
1.0
49
3.0 ± 0.14
1.08 ± 0.16
1.5
42
2.8 ± 0.40
0.85 ± 0.38
2.0
40
2.6 ± 0.39
0.93 ± 0.45
2.5
35
2.0 ± 0.81
0.49 ± 0.10
3.0
19
1.2 ± 0.22
0.32 ± 0.28
Note: mean separation was analyzed by ANOVA using SPSS software (var. 16.0) and the values represented in corresponding column followed by same letters are not significantly different according to DMRT at .
