Direct Organogenesis from Rhizome Explants in Marsilea quadrifolia L.: A Threatened Fern Species

Figure 1

(a) Regeneration of leaflets from the rhizome explants on MS medium. (b) Early stage of multiplication (stunted growth of fronds). (c) Development of multiple sporophytes on half strength MS medium without growth hormones. (d and e) In vitro propagated sporophytes with fronds and roots. (f) Hardening of sporophytes of M. quadrifolia in soilrite. (g) Hardened sporophytes growing in the fern house.

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