Interaction between Cowpea Aphid-Borne Mosaic Virus Isolates and Its Effect on Passion Fruit Woodiness Disease on Passiflora edulis Sims and Passiflora ligularis Juss
Table 4
Determination of CABMV isolate distribution in P. edulis by DAS-ELISA, absorbance value for CABMV assay indicates the presence or absence of the virus in different organs.
Plant part
Virus type
Mean absorbance value
Reaction to antisera
Leaves
CABMV 1
0.229
Positive
0.14
Negative
0.141
Negative
0.2145
Positive
CABMV 2
0.3015
Positive
0.4895
Positive
0.239
Positive
0.2545
Positive
Mixed
0.1505
Negative
1.165
Positive
0.1925
Positive
0.683
Positive
Control
0.21275
Stem
CABMV 1
0.3015
Positive
0.4895
Positive
0.239
Positive
0.2545
Positive
CABMV2
0.1325
Positive
0.373
Positive
0.159
Negative
0.1995
Positive
Mixed
0.2405
Positive
0.164
Negative
0.283
Positive
0.1565
Negative
Control
0.186
Roots
CABMV 1
0.164
Negative
0.1335
Positive
0.2255
Positive
0.1425
Negative
CABMV 2
0.288
Positive
0.3965
Positive
0.3315
Positive
0.1485
Negative
Mixed
0.146
Negative
0.157
Negative
0.149
Negative
0.411
Positive
Control
0.225
Note. Absorbance value at 450 nm. Samples with mean absorbance values higher than two times the negative control average (healthy plant parts) were considered positive.