Research Article

Interaction between Cowpea Aphid-Borne Mosaic Virus Isolates and Its Effect on Passion Fruit Woodiness Disease on Passiflora edulis Sims and Passiflora ligularis Juss

Table 4

Determination of CABMV isolate distribution in P. edulis by DAS-ELISA, absorbance value for CABMV assay indicates the presence or absence of the virus in different organs.

Plant partVirus typeMean absorbance valueReaction to antisera

LeavesCABMV 10.229Positive
0.14Negative
0.141Negative
0.2145Positive
CABMV 20.3015Positive
0.4895Positive
0.239Positive
0.2545Positive
Mixed0.1505Negative
1.165Positive
0.1925Positive
0.683Positive
Control0.21275

StemCABMV 10.3015Positive
0.4895Positive
0.239Positive
0.2545Positive
CABMV20.1325Positive
0.373Positive
0.159Negative
0.1995Positive
Mixed0.2405Positive
0.164Negative
0.283Positive
0.1565Negative
Control0.186

RootsCABMV 10.164Negative
0.1335Positive
0.2255Positive
0.1425Negative
CABMV 20.288Positive
0.3965Positive
0.3315Positive
0.1485Negative
Mixed0.146Negative
0.157Negative
0.149Negative
0.411Positive
Control0.225

Note. Absorbance value at 450 nm. Samples with mean absorbance values higher than two times the negative control average (healthy plant parts) were considered positive.