Detection of Salmonella enterica subsp. enterica via Quenching of Unincorporated Amplification Signal Reporters in Loop-Mediated Isothermal Amplification

Table 3

In vitro specificity of the negative and positive control panels. Specificity testing included various available samples and strains containing the target sequence (positive control panel) as well as samples containing nucleic acids that were expected to present negative results in the assay (negative control panel).

Negative control panel
Organism	Sample code	Accession number	Source	Characteristics
Escherichia coli	Ec	BIOTECH 1634	PNCM	Gram-negative bacterium, grown in nutrient agar
Staphylococcus aureus	Sa	BIOTECH 1582	PNCM	Gram-positive bacterium, grown in nutrient agar
Staphylococcus epidermidis	Se	BIOTECH 10098	PNCM	Gram-positive bacterium, grown in nutrient agar
Pseudomonas aeruginosa	Pa	BIOTECH 1335	PNCM	Gram-negative bacterium, grown in nutrient agar
Klebsiella pneumoniae	Kp	BIOTECH 1754	PNCM	Gram-negative bacterium, grown in nutrient agar
Enterococcus faecalis	Ef	BIOTECH 10348	PNCM	Gram-positive bacterium, grown in de man, rogosa and sharpe agar
Listeria monocytogenes	Lm	BIOTECH 1958	PNCM	Gram-positive bacterium, grown in nutrient agar
Positive control panel (Salmonella enterica subsp. enterica serovars)
Organism	Sample code	Accession number	Source	Characteristics
Salmonella enterica sv. Typhimurium	SNT	ATCC 13311	Microbiologics, inc., MN, USA	Nontyphoidal
Salmonella enterica sv. Paratyphi A	SPA	ATCC 9150	NSRI	Typhoidal
Salmonella enterica sv. Paratyphi B	SPB	ATCC 8759	NSRI	Typhoidal
Salmonella enterica sv. Typhi	STY	BIOTECH 1768	NSRI	Typhoidal

PNCM - Philippine National Collection of Microorganisms, National Institute of Molecular Biology and Biotechnology, Laguna, Philippines. NSRI - Natural Science Research Institute, University of the Philippines Diliman, Quezon City, Philippines.