Two-Component Signal Transduction System SaeRS Positively Regulates Staphylococcus epidermidis Glucose Metabolism

Table 1

Oligonucleotide primers used for RT real-time PCR.

Target gene	Gene description	GenBank accession no.	Primer*	Primer sequence	Location	Function
gyrB	DNA gyrase, B subunit	57636585	gyrBF; gyrBR	CTTATATGAGAATCCATCTGTAGG; AGAACAATCTGCCAATTTACC	1110–1263	House keeping gene
pta	Phosphate acetyltransferase	57866177	ptaF; ptaR	AGGTTCTGCTAAATCGGATGATG; GGTGCTTTCTTCTCTGCTACG	767–922	Catalyzes the conversion of acetyl-CoA and phosphate to CoA and acetyl phosphate
gapA-1	Glyceraldehyde 3-phosphate dehydrogenase	57866427	gapA-1F; gapA-1R	ACACAAGACGCACCTCAC; TCTGGAATAACTTTACCGATAGC	709–821	Catalyzes the formation of 1,3-bisphosphoglycerate from glyceraldehyde-3-P in glycolysis
eno	Enolase; phosphopyruvate hydratase	57866440	enoF; enoR	GCCAATTATTACAGATGTTTACG; CAGTAGAAGCACCAGAAGG	200–327	Catalyzes the formation of phosphoenolpyruvate from 2-phospho-D-glycerate in glycolysis
sucD	Succinyl-CoA synthetase alpha subunit	57866714	sucDF; sucDR	AGAAGAAGAAGCGGCACAATG; GCAGTTTCAACACCACAATCG	779–958	Catalyzes the only substrate-level phosphorylation in the TCA cycle
icd	Isocitrate dehydrogenase	57867183	icdF; icdR	ACTATATCTCTGATGCTCTTG; TTCTGATGACGGATTAACC	1118–1272	Converts isocitrate to alpha ketoglutarate
pyk	Pyruvate kinase	57867187	pykF; pykR	CGAGTTTCAGTGTTAGGTC; CGTAGAGGTTAATTGATTATTCC	4–159	Catalyzes the formation of pyruvate from phosphoenolpyruvate in glycolysis
fbaA	Fructose-bisphosphate aldolase	57867608	fbaAF; fbaAR	TGCTGATGGCGTTATCTATG; CCGTGTAATACTAAAGGTAATCC	575–757	Catalyzes the formation of glycerone phosphate and glyceraldehyde 3-phosphate from fructose 1,6-bisphosphate

∗F: forward primer; R: reverse prime.