Optimization of Aeration and Agitation Rate for Lipid and Gamma Linolenic Acid Production by Cunninghamella bainieri 2A1 in Submerged Fermentation Using Response Surface Methodology

Table 3

Experimental design and test results of biomass concentration, lipid yield, and GLA yield produced by Cunninghamella bainieri 2A1 in the submerged bioreactor.

Run			Biomass concentration (g/L)		Lipid yield (%)		GLA yield (g/g)
			Actual value	Predicted value	Actual value	Predicted value	Actual value	Predicted value
1	−1	1	11.35	11.35	37.28	39.06	0.05	0.052
2	0	0	11.94	11.82	38	37.75	0.048	0.049
3	0	−1.41	4.80	4.61	25.79	24.65	0.03	0.028
4	0	0	11.82	11.82	39	37.75	0.051	0.049
5	1	−1	7.17	7.38	32.79	34.27	0.045	0.047
6	0	0	11.47	11.82	36.48	37.75	0.047	0.049
7	1.41	0	12.06	11.82	39.33	37.43	0.062	0.060
8	0	0	11.82	11.82	39.17	37.75	0.05	0.049
9	1	1	12.42	12.55	31.85	34.01	0.05	0.052
10	−1.41	0	8.67	8.49	34.81	33.45	0.05	0.048
11	0	0	11.94	11.82	36.12	37.75	0.048	0.049
12	0	1.41	11.82	11.70	37.52	35.41	0.048	0.046
13	−1	−1	5.0	5.15	22.5	23.59	0.029	0.031

: aeration rate (vvm); : agitation rate (rpm).