Differentiated cells. Tumour stem cells from glioblastoma differentiate into cells expressing aquaporins 1, 4, and 9 in addition to mature neuronal and glial markers. Yet, they retain their expression of immature markers. The cells displayed obviously aberrant morphology; note the multiple nuclei. (a, b): Differentiated cells of both glial and neuronal morphology stained positively for aqp4 (green). (c) Cells also exhibited extensive positive signal for aqp9 (red). (d) Nestin positive cells (green). (e) Cells positive for TuJ1 (red) and GFAP (green) Some cells display labelling with both antigens (green and red, together). (f) Most differentiated cells were positive for aqp1 (red). Scale bars: 20 μm.
