Optimization and Validation of ELISA for Aflatoxin B1 Detection in Fermented Forages and Feeds

Table 2

Calculated ELISA data for the limit of the detection and quantification with Romer AgraQuant® aflatoxin B1 ELISA test kit (n = 8).

Parameters	Corn silage	Rye haylage	Milking cow feed concentrate
Mean concentration of the blanks (MC) (ppb)	2,146	2,040	2,135
Standard deviation (SD) (ppb)	0,099	0,102	0,098
Limit of detection (LOD) (ppb)	2,443	2,346	2,429
Limit of quantification (LOQ) (ppb)	3,037	2,958	3,017