Research Article

Optimization of Fermentation Conditions and Media for Production of Glucose Isomerase from Bacillus megaterium Using Response Surface Methodology

Table 4

Experimental design by RSM for D-xylose concentration, yeast extract concentration, and cultivation temperature to optimize glucose isomerase activity and experimental results.

TrialsVariablesEnzyme activity (U/mg biomass)
X1X4X8Experimental results

N10.50.75350.238
N21.50.75350.233
N30.51.25350.257
N41.51.25350.264
N50.50.75390.222
N61.50.75390.229
N70.51.25390.236
N81.51.25390.263
N90.51370.247
N101.51370.271
N1110.75370.253
N1211.25370.277
N1311350.269
N1411390.267
N1511370.279
N1611370.276
N1711370.280