Optimization of Fermentation Conditions and Media for Production of Glucose Isomerase from Bacillus megaterium Using Response Surface Methodology
The effect of the concentration of peptone on glucose isomerase production. Peptone is the second nitrogen source for bacterial growth and enzyme synthesis; therefore, the concentration of peptone may affect the glucose isomerase activity. In this study, the concentration of peptone was increased from 0 to 1% along with an elevation of enzyme activity from 0.182 ± 0.002 to 0.263 ± 0.004 U/mg biomass (). Then, the enzyme activity decreased along with the higher increase of peptone concentration (1.5–2.5%). Therefore, we chose 1% peptone as the optimal concentration to produce glucose isomerase.
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