Optimization of Fermentation Conditions and Media for Production of Glucose Isomerase from Bacillus megaterium Using Response Surface Methodology

<div>The effect of the concentration of MgSO4·7H2O on glucose isomerase production. The concentration of MgSO4·7H2O was increased (0-0.1%) along with enhancement of enzyme activity. We observed that the higher concentration of MgSO4·7H2O (0.15–0.25%) resulted in a decline of enzyme activity. We chose 0.1% of MgSO4·7H2O to further optimization experiments. a, b, c, and d indicate the significant difference between groups.</div>

Scientifica

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Figure 4

Figure 4: Optimization of Fermentation Conditions and Media for Production of Glucose Isomerase from Bacillus megaterium Using Response Surface Methodology

Figure 4 | Optimization of Fermentation Conditions and Media for Production of Glucose Isomerase from Bacillus megaterium Using Response Surface Methodology 