Optimization of Fermentation Conditions and Media for Production of Glucose Isomerase from Bacillus megaterium Using Response Surface Methodology
The effect of the concentration of K2HPO4 on glucose isomerase production. Enzyme activity was significantly increased after supplement with 0.2% of K2HPO4 as compared to medium without K2HPO4. However, we observed a decline of enzyme activity while the concentration of K2HPO4 was adjusted from 0.3 to 0.5% (0.225 ± 0.002 and 0.207 ± 0.005 U/mg biomass, accordingly, ). We chose the optimal concentration of K2HPO4 is 0.2%. a, b, c, and d indicate the significant difference between groups.
You are browsing a BETA version of Hindawi.com. Click here to switch back to the original design.