Site Saturation Mutagenesis Applications on Candida methylica Formate Dehydrogenase

Table 2

Activity and values of site saturation mutants on Met1 position of cmFDH.

cmFDH Met1 mutants	(mM)	(s^−1)	/ (s^−1 mM^−1)
Native cmFDH	4.75 ± 0.3	1.1 ± 0.1	0.24	56.7 ± 0.2
Met1Cys	3.3 ± 0.4	1.25 ± 0.005	0.4	58.4 ± 0.2 [7]
Met1QGln	2.9 ± 0.4	0.4 ± 0.002	0.13	54.65 ± 0.2
Met1Gly	2.6 ± 0.3	0.1 ± 0.0005	0.04	53.2 ± 1.3
Met1Asn	2.8 ± 0.3	0.3 ± 0.001	0.1	53.3 ± 1.8
Met1Ala	4.02 ± 0.45	0.4 ± 0.002	0.1	55.6 ± 0.7
Met1Arg	9.8 ± 4.5	0.6 ± 0.01	0.06	55.8 ± 3
Met1Val	3.3 ± 0.2	0.5 ± 0.001	0.16	52.55 ± 0.5
Met1Ser	4.5 ± 0.5	0.9 ± 0.004	0.2	55.1 ± 0.7
Met1Leu	6.3 ± 1	1.2 ± 0.008	0.18	56.6 ± 0.2