Molecular Modulation of Fetal Liver Hematopoietic Stem Cell Mobilization into Fetal Bone Marrow in Mice

Figure 2

Notch1 transcriptional activation domain is essential for HSC expansion in the mouse fetal liver. Upon Notch1 activation, Notch1 intracellular domain (Notch1-ICN) gets into the nucleus forming complex with RBPJ and MAML, which increases expression of Notch1 downstream target genes and fetal HSC expansion. Loss of Notch1 transcriptional activation domain (Notch1ΔTAD/ΔTAD) leads to the failure of formation of Notch1-ICN/RBPJ/MAML transcription complex, which decreases the expression of Notch1 downstream target genes, resulting in HSC apoptosis.