Stem Cells International

Research Article

Comparative Analysis of Biological Properties of Large-Scale Expanded Adult Neural Crest-Derived Stem Cells Isolated from Human Hair Follicle and Skin Dermis

Table 1

Growth parameters of adult NCSCs cultures originated from human hair follicles and skin dermis.


Cell line	P0		P1				P2				P3				CFU frequency at P3 (%)
Cell line	Cell culture vessel	Cell yield (×10⁶)	Cell culture vessel	Cell yield (×10⁶⁾	PDN	PDT (hours)	Cell culture vessel	Cell yield (×10⁶)	PDN	PDT (hours)	Cell culture vessel	Cell yield (×10⁶)	PDN	PDT (hours)	CFU frequency at P3 (%)

HF NCSCs 1	2 × Petri dish, ø 35 mm	0.183	T25 flask	1.355	5.74	29.27	5-layer multiflask	44.394	5.64	29.79	5-layer multiflask	42.155	5.57	30.16	22.4
HF NCSCs 2	2 × Petri dish, ø 35 mm	0.142	T25 flask	0.93	5.20	32.31	5-layer multiflask	39.462	5.46	30.77	5-layer multiflask	41.647	5.55	30.27	26.8
HF NCSCs 3	2 × Petri dish, ø 35 mm	0.225	T25 flask	1.265	5.64	29.79	5-layer multiflask	42.727	5.59	30.05	5-layer multiflask	40.032	5.5	30.55	20.5
HF NCSCs 4	2 × Petri dish, ø 35 mm	0.114	T25 flask	1.428	5.81	28.92	5-layer multiflask	51.215	5.85	28.72	5-layer multiflask	44.509	5.65	29.73	31.7
HF NCSCs 5	2 × Petri dish, ø 35 mm	0.163	T25 flask	1.173	5.53	30.38	5-layer multiflask	47.605	5.75	29.22	5-layer multiflask	43.584	5.62	29.89	28.2

Mean		0.165		1.230	5.584	30.132		45.081	5.658	29.709		42.385	5.578	30.121	25.92
SD		0.042		0.193	0.239	1.336		4.516	0.150	0.786		1.738	0.059	0.318	4.50
SD NCSCs 1	T25 flask 3D gel	2.192	5-layer multiflask	56.284	5.99	28.05	5-layer multiflask	58.544	6.04	27.81	5-layer multiflask	53.057	5.90	28.47	31.5
SD NCSCs 2	T25 flask 3D gel	2.517	5-layer multiflask	59.318	6.06	27.72	5-layer multiflask	62.05	6.13	27.41	5-layer multiflask	60.827	6.10	27.54	33.2
SD NCSCs 3	T25 flask 3D gel	3.207	5-layer multiflask	66.436	6.22	27.01	5-layer multiflask	64.719	6.19	27.14	5-layer multiflask	62.586	6.14	27.36	38.4
SD NCSCs 4	T25 flask 3D gel	1.458	5-layer multiflask	48.375	5.77	29.12	5-layer multiflask	54.894	5.95	28.24	5-layer multiflask	52.455	5.88	28.57	30.9
SD NCSCs 5	T25 flask 3D gel	2.372	5-layer multiflask	62.085	6.13	27.41	5-layer multiflask	55.325	5.96	28.19	5-layer multiflask	61.095	6.10	27.54	36.5

Mean		2.349		58.500	6.034	27.860		59.106	6.054	27.757		58.004	6.024	27.898	34.10
SD		0.629		6.781	0.170	0.800		4.258	0.105	0.480		4.842	0.124	0.576	3.24