Human Adipose-Derived Stem Cells Promote Seawater-Immersed Wound Healing by Activating Skin Stem Cells via the EGFR/MEK/ERK Pathway

Figure 2

Effect of hADSCs on the proliferation and migration of SW-treated cells in vitro. CCK8 assay results showed that the proliferation of HaCaT cells was significantly inhibited following exposure to 3.5% salt concentration (a). Transwell coculture system schematic (b). The cell scratch test (c) showed that the SW group showed a significant closure delay at the 12th hour compared with the control group, and the hADSC coculture reduced the effect of SW on cell migration ability and significantly promoted cell migration (d). Proliferating HaCaT cells were stained with green fluorescence, and all nuclei were stained with blue fluorescence (e). The results of the EdU assay showed that the cell proliferation rate in the SW group was significantly inhibited compared with that in the control group and the SW+hADSC group (f). Scale bars indicate 100 μm. , , and .

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