Activator Protein-1 Transcriptional Activity Drives Soluble Micrograft-Mediated Cell Migration and Promotes the Matrix Remodeling Machinery

Figure 4

AP-1 in MG-treated cells does not affect fibroblast cell proliferation and viability. (a, b) Cell cycle changes were analyzed using the common 5-ethynyl-2-deoxyuridine (EdU) assay (associated with DAPI staining to evaluate the nuclear content) on both MG-treated fibroblasts and human keratinocytes exposed to 5 and 12 h of MG treatment in the presence or absence of the AP-1 inhibitor T-5224. Quantification average of is shown. Data is presented as the . Two-way ANOVA was used to perform statistical analysis. ; ; . (c, d) MG-induced effects on cell viability were evaluated via FACS analysis using the Fixable Viability Stain 660. Percentage (%) of viable cells after exposure to 5 and 12 h of MG treatment is shown. No significant differences were found among the different conditions.

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