The Effect of Blood-Derived Products on the Chondrogenic and Osteogenic Differentiation Potential of Adipose-Derived Mesenchymal Stem Cells Originated from Three Different Locations

Table 2

Primers used in the real-time polymerase chain reaction.

	Amplicon (bp)	Annealing temp. (°C)
Osteogenic markers
GAPDH	143	60
Forward: 5-ACATCGCTCAGACACCATG-3
Reverse: 5-TGTAGTTGAGGTCAATGAAGGG-3
ALPL	133	59
Forward: 5-GATGTGGAGTATGAGAGTGACG-3
Reverse: 5-GGTCAAGGGTCAGGAGTTC-3
RUNX2	148	61
Forward: 5-TTCACCTTGACCATAACCGTC-3
Reverse: 5-GGCGGTCAGAGAACAAACTAG-3
COL1A1	167	62
Forward: 5-TCCAAACCACTGAAACCTCTG-3
Reverse: 5-CCCCTGGAAAGAATGGAGATG-3
OCN	106	59
Forward: 5-GGCGCTACCTGTATCAA TGG-3
Reverse: 5-TCAGCCAACTCGTCACA GTC-3
Chondrogenic markers
GAPDH	112	60
Forward: 5-CTCTGCTCCTCCTGTTCGAC-3
Reverse: 5-ACGACCAAATCCGTTGACTC-3
COL1A1	63	61
Forward: 5-GGGATTCCCTGGACCTAAAG-3
Reverse: 5-GGAACACCTCGCTCTCCAG-3
COL2AB	116	62
Forward: 5-GCACCTGCAGAGACCTGA-3
Reverse: 5-GGGTCAATCCAGTAGTCTCCAC-3
SOX9	73	59
Forward: 5-TACCCGCACTTGCACAAC-3
Reverse: 5-TCTCGCTCTCGTTCAGAAGTC-3
MMP3	91	58
Forward: 5-CAAAACATATTTCTTTGTAGAGGACAA-3
Reverse: 5-TTCAGCTATTTGCTTGGGAAA-3