Lung Mesenchymal Stem Cells Ameliorate Elastase-Induced Damage in an Animal Model of Emphysema

Figure 1

MSC characterization and engraftment. (a) Immunophenotypic profile by flow cytometry of MSCs isolated from adult mouse lungs. Grey-shaded peaks show CD markers; red histograms represent isotype control. (b) Representative image of MSCs after lentiviral transduction of GFP (green). (c, d) In vivo engraftment of GFP-positive MSCs (green) in emphysematous lungs ten days after intratracheal cell administration. (e) Representative image of GFP-positive MSCs (green) and CD45-positive cells (red). (f) Negative control for GFP staining in a PPE lung. Nuclei are counterstained with DAPI (blue). Scale bars: 20 μm (d, e), 50 μm (b, f), and 100 μm (c).

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