Comparison of Immunological Characteristics of Mesenchymal Stem Cells from the Periodontal Ligament, Umbilical Cord, and Adipose Tissue

Figure 1

Characterization of human MSCs derived from the periodontal ligament (PDLSC), from the umbilical cord (UC-MSCs), and from the adipose tissue (AD-MSCs). (a) Cells were cultured for five to eight passages, harvested, labeled with antibodies against CD34, CD45, CD73, CD90, or CD105, and analyzed by flow cytometry. Black histograms indicate negative controls, and blue histograms represent PDLSCs stained with the indicated antibodies. (b) Cells were induced to differentiate into adipocytes, chondrocytes, or osteoblasts using the appropriate culture medium. Oil Red O, Safranin O, or Alizarin Red staining of adipocytes, chondrocytes, or osteoblasts was performed on days 14, 21, or 28 postinduction, respectively. All experiments were performed independently at least three times, and representative figures are shown.

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