Human Adipose Mesenchymal Stem Cells Show More Efficient Angiogenesis Promotion on Endothelial Colony-Forming Cells than Umbilical Cord and Endometrium

Figure 3

Comparison of in vitro proangiogenic potential of ADSCs, UCMSCs, and EMSCs. (a) Tubular-like structures formed by ECFCs at the presence of different MSCs on matrigel, respectively (×100). ECFCs and MSCs were cocultured on matrigel-coated culture plate; tubular-like networks were shown in different groups after 2 hours. ECFCs were labeled by UEA-I in red and MSCs by CFSE in green. The group of ECFC was used as control. (b) Tubular-like structures formed by ECFCs directly cultured on the monolayer of different MSCs, respectively (×40). MSCs were first seeded on culture dishes, and ECFCs were then seeded on the MSCs layers after 24 h. ECFCs were labeled by UEA-I in red. Single ECFCs were used as control. (c) Statistical analysis of vascular length, vascular junctions, and ratio of vascular area formed by ECFCs in (b). The data were determined using ImageJ software. Bars represent mean value ± SD. , , compared with ECFC group; , compared with ECFC + ADSC group. .

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