Comparative Analysis of Spontaneous and Stimulus-Evoked Calcium Transients in Proliferating and Differentiating Human Midbrain-Derived Stem Cells

Figure 7

The fraction of proliferating cells with calcium transients during 15 min of incubation. Representative calcium traces from cells kept under six different experimental conditions (trace from five cells in each group) (a). The cells were loaded with 5 μM Fluo-4. Calcium was present during the loading and the incubation procedures as indicated on the figure. Thapsigargin was used for depletion of calcium stores. Thapsigargin was present during the loading procedure. Carbachol and pirenzepin were present during the incubation. Sodium-free solution was used only for the incubation. Mean values and SEM, 6–14 independent experiments (b).

(a)

(b)