Isolation and Characterization of Synovial Mesenchymal Stem Cell Derived from Hip Joints: A Comparative Analysis with a Matched Control Knee Group

Figure 2

(a) Arthroscopic observation and sample harvesting. (b), (c) Macroscopic features of from knee and hip synovium were examined on a 1 mm scare and in PBS. (d), (e) Histological analysis was performed on tissues stained with hematoxylin and eosin (HE). (f) Representative cell colonies derived from synovium of a donor stained with crystal violet showing large colonies when the nucleated cells were plated at 10⁴ cells/dish. There was no significant difference in the number of colonies between both MSCs from knee and hip joints. (g) Representative morphologic features of the cells magnified at 14 days (passage 0) showing fibroblastic spindle cell morphology. Bar = 100 μm. (h) Representative cell colonies stained with crystal violet derived from the synovial fluid of a donor showing large colonies from the knee, but no colonies from the hip.

(a)

(b)

(c)

(d)

(e)

(f)

(g)

(h)