Research Article
Hepatoma-Derived Growth Factor Secreted from Mesenchymal Stem Cells Reduces Myocardial Ischemia-Reperfusion Injury
Figure 3
HDGF contributed to the protective effects of MSC-CdM. Conditioned medium that collected from MSCs transfected with vector (MSCnull-CdM) or HDGF shRNA lentivirus (MSCHDGF KD-CdM) were treated to H9C2 cells subjected to 9 h of hypoxia followed by 4 h of reoxygenation. (a) Cell death was evaluated with flow cytometry analysis. Annexin V−/PI−: viable cells; Annexin V+/PI−: early apoptotic cells; Annexin V+/PI+: late apoptotic or necrotic cells; Annexin V−/PI+: necrotic cells. (b) Cleaved caspases-3 as detected by Western blotting. Data represent mean ± SEM of values from three determinations. .
(a) |
(b) |