Research Article
Hepatoma-Derived Growth Factor Secreted from Mesenchymal Stem Cells Reduces Myocardial Ischemia-Reperfusion Injury
Figure 1
MSC-CdM reduce cardiac reperfusion injury. Wild-type mice were given 5 mg/kg CF-CdM, 5 mg/kg MSC-CdM, or vehicle i.v. 15 min before 45 min of ischemia. MSC-CdM: conditioned medium derived from MSC; CF-CdM: conditioned medium derived from cardiac fibroblasts. (a) Ratio of area at risk (AAR) to left ventricular (LV) area, ratio of infarct size (I) to AAR, and ratio of infarct size to LV after 24 h of reperfusion. Data represent mean ± standard error of mean (SEM) of values from five mice. (b) The maximum rates of rise and decline of left-ventricular pressure (+dp/dtmax and −dp/dtmax) assessed at 24 h reperfusion. Data are mean ± SEM of values from six mice. (c) TUNEL staining at 24 h reperfusion; apoptotic nuclei were stained (red), and cardiomyocytes were detected by cardiac troponin I staining (green). Bar = 50 μM. Data are mean ± SEM of values from three hearts per group, with at least 3000 nuclei examined per heart. .
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