Clinical Observation of Employment of Umbilical Cord Derived Mesenchymal Stem Cell for Juvenile Idiopathic Arthritis Therapy

Figure 3

Cell quality of MSCs. MSCs were analyzed with agar cloning assay, acridine orange staining, and hoechst staining, respectively. In the cloning assay, MSCs showed no significant clones compared with HeLa (a). Acridine orange staining showed that only MSCs were stained indicating no fungal contamination (b). Hoechst 33258 staining showed that only MSCs were stained suggesting no mycoplasma contamination (c). This result was taken from one of the ten samples for patients.

(a)

(b)

(c)