A Controlled Release Codelivery System of MSCs Encapsulated in Dextran/Gelatin Hydrogel with TGF-β3-Loaded Nanoparticles for Nucleus Pulposus Regeneration

Table 1

Real-time polymerase chain reaction primers.

Gene	Sequence	Size
Aggrecan	Forward: 5′ ATTTCCACACGCTACACCCTG 3′	164 bp
	Reverse: 5′ TGGATGGGGTATCTGACTGTC 3′
Type II collagen α1	Forward: 5′ CAGGATGCCCGAAAATTAGGG 3′	132 bp
	Reverse: 5′ ACCACGATCACCTCTGGGT 3′
KRT18	Forward: 5′ TCCATCAGGGTGACTCAGAAA 3′	250 bp
	Reverse: 5′ CCAGCTTCAAGGGGCTCAA 3′
Shh	Forward: 5′ AAAGCTGACCCCTTTAGCCTA 3′	123 bp
	Reverse: 5′ TTCGGAGTTTCTTGTGATCTTCC 3′
GAPDH	Forward: 5′ GGAGTTGCTGTTGAAGTCGCA 3′	532 bp
	Reverse: 5′ GGAGTTGCTGTTGAAGTCGCA 3′

Primers for aggrecan, type II collagen α1, Shh, and GAPDH were designed from mus musculus gene sequences obtained from the NCBI GenBank and RefSeq databases using primer 5.0 software.