Effective Mobilization of Very Small Embryonic-Like Stem Cells and Hematopoietic Stem/Progenitor Cells but Not Endothelial Progenitor Cells by Follicle-Stimulating Hormone Therapy

Figure 2

Representative FACS plots demonstrating the gating strategy for CD34⁺ cells and EPCs. PBMCs were gated based on forward and side scatter (FSC/SSC) plot (b). CD34⁺ cells were gated on CD34/SSC plot (c). CD34⁺CD144⁺ cells (upper right quadrant, Q2) were gated based on CD34/CD144 dot plot (d). FMO control was used to set the gates (a). In order to determine CD34⁺CD144⁺CD309⁺ cell numbers, the PBMC events were displayed on the basis of FMO control sample (e) and gates were set to exclude the random noise. Next, the gates were visualized on CD34/CD133 plot (f) and CD34⁺CD133⁺ cells were gated (upper right quadrant, Q2). Finally, CD34⁺CD133⁺CD309⁺ cells subsets were gated on histogram plot (g) (black peak, control; grey peak, positive staining).