Magnet-Bead Based MicroRNA Delivery System to Modify CD133⁺ Stem Cells

Figure 7

Haematopoietic differentiation capacity of cells after transfection. Cells were transfected with optimized miR/PEI (20 pmol miR; N/P ratio 7.5) and miR/PEI/MNP (20 pmol miR; N/P ratio 7.5; 3 and 5 μg/mL MNPs) complexes and colony-forming unit (CFU) assays were performed. After incubation for 14 d, CFU-erythroid (CFU-E) (a), Burst-forming unit-erythroid (BFU-E) (b), CFU-granulocyte, erythroid, macrophage, and megakaryocyte (CFU-GEMM) (c), and CFU-granulocyte and macrophage (CFU-GM) (d) were counted (e). Presented pictures are illustrating observed cell morphologies. Untransfected cells were used as control. Values are presented as mean ± SEM; ; statistic was performed within respective units; ; ; . Scale bar = 200 μm.

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