Magnet-Bead Based MicroRNA Delivery System to Modify CD133+ Stem Cells
Magnetic targeting of modified CD133+ cells. Cells were transfected with Cy3-labeled complexes consisting of 20 pmol miR, two different N/P ratios (5 and 7.5), and MNP amounts (3 and 5 μg/mL). 18 h after transfection, a local magnetic field was applied for 24 h. Pictures were taken from areas with and without magnet (a). Cell numbers in both areas were counted (c) and magnetic targeting ratios were calculated (b). Untransfected cells were used as control. Values are presented as mean ± SEM; ; statistic was performed versus control (indicated as ∗) or within respective MNP amounts (indicated as #); ; ; . Scale bar = 100 μm.
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