Ciprofloxacin Improves the Stemness of Human Dermal Papilla Cells

Figure 2

Effects of CIP on stem cell-like characteristics in DPCs. (a) Cells were treated with CIP (0–10 μg/mL) for various times (0–72 h). After indicated treatment, morphology of DPCs was observed. Scale bar is 100 μm. (b) Aggregation behavior of cells was determined after indicated treatment for 72 h. Scale bar is 100 μm. ((c)-(d)) Aggregation size and aggregation number were determined by image analyzer. The data represent the means of four independent samples ± SD. versus untreated control. ((e)-(f)) Cells were cultured in the presence or absence of CIP (10 μg/mL) for 72 h and serum-starved for 24 h. After serum-starvation, cells were incubated with complete media for 12 h. The cells at the early passages (passages 2-3) without serum-starvation were also used as an untreated control at 0 h. Cell cycle distribution was determined by PI staining and flow cytometry. The data represent the means of four independent samples ± SD. versus untreated control at 0 h; ^# versus untreated control at 72 h.

(a)

(b)

(c)

(d)

(e)

(f)