Genetically engineered mutant of alpha-hemolysin from Staphylococcus aureus was used to create pores in the cellular membrane of 3T3 fibroblasts and human keratinocytes. Resulting nonselective pore is equipped with a metal-actuated switch that is sensitive to extracellular zinc concentrations, thus permitting controlled loading of trehalose
Human MSCs were loaded by trehalose up to 30 mM internal concentration at usual cultivation conditions for 24 h at 37 in the presence of MSC medium with 0–125 mM trehalose