Human Adipose-Derived Mesenchymal Stem Cells Cryopreservation and Thawing Decrease α4-Integrin Expression

Figure 5

Osteogenic differentiated cells after 35 days in osteogenic induction medium: sample after thawing of cryopreserved cells, 250x rise. (a) ADMSC stained with Alizarin Red, demonstrating the presence of calcium in extracellular matrix. (b) Control without matrix demonstration, indicating the undifferentiated cells cultivated with standard culture medium. Scale (10 μm).

(a)

(b)