Research Article

Exosomes from Human Umbilical Cord Mesenchymal Stem Cells: Identification, Purification, and Biological Characteristics

Figure 4

HucMSCs-exosomes promote cell proliferation under oxidative stress. (a) Immunohistochemical staining of proliferative cell nuclear antigen. H9C2(2-1), HL-7702, and NRK-52E cells were pretreated with hucMSCs-exosomes (800 μg/mL) for 24 hours, followed by exposure to H2O2 (300, 300, and 500 μM) for different time points (24, 6, and 6 hours). (A–C) H9C2(2-1); (D–F) HL-7702; and (G–I) NRK-52E. Control (A, D, and G); H2O2 (B, E, and H); and hucMSCs-exosomes (C, F, and I). Scale bar: 100 μm. (b) Quantitative analysis of the percentage of PCNA-positive cells. and compared to H2O2 group.
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