Research Article

Inhibition of G9a Histone Methyltransferase Converts Bone Marrow Mesenchymal Stem Cells to Cardiac Competent Progenitors

Figure 5

Shared expression of progenitor markers in atrial and bone marrow-derived PBCs. PBCs were collected from either (a–e) atrial cultures or (f–j) BIX01294 plus CCM-treated MSCs and immunostained following (a–c; e–h, i) their attachment on Cell-Tak coated chamber slides or (d, i) being cytospun onto glass slides. Subsequent immunofluorescent labeling of the cells (shown in gray) was used to detect the expression of (a, f) Sca-1 (b, g) CD90 (c, h) Isl-1, (d, i) Cx43, and (e, j) Cx40. Cultures were counterstained with DAPI to visualize nuclei (blue). Scale bar = 25 μm.
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