Selection of Reference Genes for Quantitative Gene Expression in Porcine Mesenchymal Stem Cells Derived from Various Sources along with Differentiation into Multilineages

Figure 3

Confirmation of primer specificity and amplicon size in various MSCs with selected reference genes. Each melting curve analysis of twelve selected reference genes was presented (top of individual genes) and each product of amplification was shown by agarose gel electrophoresis (bottom of individual genes). Lanes, which were displayed from the left to the right, showed 100 bp ladder, undifferentiated MSCs, and differentiated cells into adipocytes or osteoblasts or chondrocytes, respectively. UN-D: undifferentiated MSCs; AD: adipogenesis; OS: osteogenesis; CH: chondrogenesis; N/C: negative control.