Characterization of Progenitor Cells during Canine Retinal Development

Table 1

Antibodies and dilutions.

Antibody	Brand	Dilution
Rabbit polyclonal to PAX6	Abcam, MA, USA (ab5790)	1 : 250
Rabbit polyclonal to Nestin	Abcam, MA, USA (ab5968)	1 : 250
Rabbit polyclonal to C-kit	Santa Cruz Biotechnology, CA, USA (sc-168)	1 : 200
Rabbit polyclonal to BDNF	Santa Cruz Biotechnology, CA, USA (sc-546)	1 : 250
Rabbit polyclonal to BRN3A	Abcam, MA, USA (ab23579)	1 : 50
Mouse monoclonal to Thy1.1	Abcam, MA, USA (ab65193)	1 : 200
Rabbit polyclonal to Histone 3 (H3)	Upstate, Temecula CA, USA (06–570)	1 : 200
Anti-rabbit IgG TRITC conjugate	Zymed, San Francisco, CA, USA (81–6114)	1 : 100
Anti-rabbit IgG Cy 5 conjugate	Zymed, San Francisco, CA, USA (81–6116)	1 : 100
Anti-rabbit IgG FITC conjugate	Zymed, San Francisco, CA, USA (81–611)	1 : 100
Anti-mouse IgG TRITC conjugate	Zymed, San Francisco, CA, USA (81–6714)	1 : 100
Anti-mouse IgG FITC conjugate	Zymed, San Francisco, CA, USA (61–6511)	1 : 100

Primary and secondary antibodies used for immunofluorescence. All dilutions were prepared in bovine serum albumin.