Research Article

Characterization of Progenitor Cells during Canine Retinal Development

Figure 3

C-kit (a), Pax6 (b) and Nestin (c) expression in the retina from fetal stage. The expression pattern was evident in the INBL. At this stage the differentiation of ganglion cells was evident as were the markers BDNF (d), Thy1 (e) and Brn3a (f). (g) Confocal image showing the Thy1 positive cells. (h) The same image was presented in (g) but made evident with H3. (i) Merged image, but the proliferation of Thy1 cells is evident, localized in INBL (arrows). (j) Merged image illustrates the colocalization of Pax6 positive cells with H3 positive cells (arrows). (k) Merged image illustrating proliferation of BDNF positive cells in the INBL made evident by H3 (yellow stained). (l) Colocalization of BDNF and Brn3a proteins distributed along the INBL (arrows).
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