Research Article
Interaction Effects of Season of Birth and Cytokine Genes on Schizotypal Traits in the General Population
Table 2
Genotyping conditions for the IL1B rs16944, IL4 rs2243250, and IL-1RN VNTR polymorphisms.
| | Polymorphism | Primer | PCR reaction mixture and program | Restriction endonuclease | PCR or digestion products |
| IL1B
rs16944 | Forward
5′TGGCATTGATCTGGTTCATC3′
reverse
5′GTTTAGGAATCTTCCCACTT3′ | 94°C/2 min
30 cycles:
94°C/20 s
50°C/20 s
72°C/20 s
72°C/4 min | Ama87I
(SybEnzym, Russia) | Digested
C allele,
190 +
115 bp,
nondigested
T allele,
305 bp |
| IL4
rs2243250 | Forward
5′ACTAGGCCTCACCTGATACG3′
reverse
5′GTTGTAATGCAGTCCTCCTG3′ | 94°C/2 min
30 cycles:
94°C/30 s
50°C/30 s
72°C/30 s
72°C/4 min | BslFI
(SybEnzym, Russia) | Digested C allele,
177 +
18 bp,
nondigested
T allele, 195 bp |
| IL-1RN
VNTR | Forward
5′-CTCAGCAACACTCCTAT-3′
reverse
5′-TCCTGGTCTGCAGGTAA3′ | 94°C/2 min
30 cycles:
94°C/20 s
50°C/20 s
72°C/20 s
72°C/4 min | - | Allele 1,
412 bp,
allele 2,
240 bp,
allele 3,
498 bp,
allele 4,
326 bp,
allele 5,
584 bp |
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